MmRPE65_L450
(Plasmid
#250725)
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PurposeBacterial and Mammalian expression of Leu450 variant Mouse RPE65
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250725 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCDNA3.1
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Vector typeMammalian Expression, Bacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMouse RPE65 L450
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SpeciesM. musculus (mouse)
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MutationA1348>C
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Entrez GeneRpe65 (a.k.a. 65kDa, A930029L06Rik, LCA2, Mord1, RP20, rd12)
- Promoter T7 / CMV
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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A portion of this plasmid was derived from a plasmid made byIDT / Ma Group
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MmRPE65_L450 was a gift from Martin Horvath (Addgene plasmid # 250725 ; http://n2t.net/addgene:250725 ; RRID:Addgene_250725)