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puDNA5_SSF_DOR_WT_APEX2
(Plasmid #250761)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250761 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    puDNA5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DOR(WT)
  • Species
    M. musculus (mouse)
  • Promoter UBC
  • Tags / Fusion Proteins
    • Signal Sequence FLAG (N terminal on insert)
    • APEX2 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    puDNA5_SSF_DOR_WT_APEX2 was a gift from Braden Lobingier (Addgene plasmid # 250761 ; http://n2t.net/addgene:250761 ; RRID:Addgene_250761)

  • For your References section:

    An engineered trafficking biosensor reveals a role for DNAJC13 in DOR downregulation. Novy B, Dagunts A, Weishaar T, Holland EE, Adoff H, Hutchinson E, De Maria M, Kampmann M, Tsvetanova NG, Lobingier BT. Nat Chem Biol. 2025 Mar;21(3):360-370. doi: 10.1038/s41589-024-01705-2. Epub 2024 Sep 2. 10.1038/s41589-024-01705-2 PubMed 39223388
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