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pHACK(Gal4)-DONR(T2A-D10A)
(Plasmid #250798)

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  • Purpose
    Drosophila transgenic vector: HACK donor vector for inserting T2A-D10A into Gal4 coding sequence by genetic crosses, converting Gal4 into a T2A-D10A transgene.
  • Depositing Lab
    Chun Han
  • Depositing Organization
    Cornell University
    Located in United States of America
  • Publication
    Shen et al bioRxiv 2025.10.30.685647 ( How to cite )
  • Sequence Information

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250798 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pACGCB(2.1)-Gal4
  • Vector type
    Insect Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    T2A-D10A
  • Species
    D. melanogaster (fly)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains D10A.

Please visit https://doi.org/10.64898/2025.12.07.692848 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHACK(Gal4)-DONR(T2A-D10A) was a gift from Chun Han (Addgene plasmid # 250798 ; http://n2t.net/addgene:250798 ; RRID:Addgene_250798)

  • For your References section:

    Tuning Mitotic Recombination with Patterned DNA Nicks for Precision Mosaic Analysis. Shen Y, Yeung AT, Wang B, Ditchfield P, Korn E, Han C. bioRxiv 2025.10.30.685647 10.1101/2025.10.30.685647
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