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pJT104
(Plasmid #25088)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 25088 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSB4A3
  • Backbone manufacturer
    BioBrick vector-Shetty et al 2008
  • Backbone size w/o insert (bp) 3443
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Edge detector delta luxI
  • Species
    E.coli
  • Insert Size (bp)
    5317

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

Addgene's quality control sequence detected a 1bp mut in LacZ ORF; it is not known to affect function

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJT104 was a gift from Christopher Voigt (Addgene plasmid # 25088 ; http://n2t.net/addgene:25088 ; RRID:Addgene_25088)
  • For your References section:

    A synthetic genetic edge detection program. Tabor JJ, Salis HM, Simpson ZB, Chevalier AA, Levskaya A, Marcotte EM, Voigt CA, Ellington AD. Cell. 2009 Jun 26. 137(7):1272-81. 10.1016/j.cell.2009.04.048 PubMed 19563759