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pENTR-EGFP-lysenin
(Plasmid #251001)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251001 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pDONR221
  • Backbone manufacturer
    Thermo Fisher Scientific
  • Backbone size w/o insert (bp) 2566
  • Total vector size (bp) 4180
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    lysenin
  • Species
    Eisenia fetida
  • Insert Size (bp)
    888
  • Mutation
    N-terminal methionine not included, W20A mutation
  • GenBank ID
    D85846
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Felix Randow, MRC Laboratory of Molecular Biology, Cambridge, UK

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-EGFP-lysenin was a gift from Jörn Coers (Addgene plasmid # 251001 ; http://n2t.net/addgene:251001 ; RRID:Addgene_251001)
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