pMVP-lenti-blast-CPAF-EF-A-mCherry
(Plasmid
#251005)
-
PurposeLentiviral vector with constitutive expression of mCherry tagged CPAF protease that is catalytically inactive
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 251005 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepMVP/Lenti/Blast-DEST
-
Backbone manufacturerChristopher Newgard (Addgene plasmid #121849)
- Backbone size w/o insert (bp) 9188
- Total vector size (bp) 10485
-
Vector typeMammalian Expression, Lentiviral
-
Selectable markersBlasticidin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameprotease-like activity factor CPAF
-
Alt namecpa
-
Alt nameCTL0233
-
SpeciesChlamydia trachomatis
-
Insert Size (bp)1734
-
Mutationtype II secretion signal deleted, amino acids 1-23, F45A, E558A (amino acid positions correspond to full length protein)
-
GenBank IDWP_009873463
-
Tag
/ Fusion Protein
- mCherry (C terminal on insert)
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pMVP-lenti-blast-CPAF-EF-A-mCherry was a gift from Jörn Coers (Addgene plasmid # 251005 ; http://n2t.net/addgene:251005 ; RRID:Addgene_251005)