pENTR-R4-LGALS3-R3
(Plasmid
#251008)
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PurposeGateway entry clone for multipart gateway assembly with human galectin 3
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 251008 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepDONR221 P4r-P3r
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Backbone manufacturerNovoPro
- Backbone size w/o insert (bp) 2660
- Total vector size (bp) 3423
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namegalectin 3
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Alt nameLGALS3
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SpeciesH. sapiens (human)
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Insert Size (bp)753
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GenBank IDNG_017089
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Entrez GeneLGALS3 (a.k.a. CBP35, GAL3, GALBP, GALIG, L31, LGALS2, MAC2)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13-F
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR-R4-LGALS3-R3 was a gift from Jörn Coers (Addgene plasmid # 251008 ; http://n2t.net/addgene:251008 ; RRID:Addgene_251008)