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pINDUCER10‐miR CNOT1
(Plasmid #251030)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251030 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pINDUCER10
  • Backbone manufacturer
    academic
  • Backbone size w/o insert (bp) 13147
  • Total vector size (bp) 13278
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    shRNA against CNOT1
  • gRNA/shRNA sequence
    Unknown
  • Species
    H. sapiens (human)
  • Entrez Gene
    CNOT1 (a.k.a. AD-005, CDC39, HPE12, NOT1, NOT1H, VIBOS)
  • Promoter tet-inducible

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer turbo RFP
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pINDUCER10‐miR CNOT1 was a gift from Lienhard Schmitz (Addgene plasmid # 251030 ; http://n2t.net/addgene:251030 ; RRID:Addgene_251030)

  • For your References section:

    The CCR4-NOT complex contributes to repression of Major Histocompatibility Complex class II transcription. Rodriguez-Gil A, Ritter O, Saul VV, Wilhelm J, Yang CY, Grosschedl R, Imai Y, Kuba K, Kracht M, Schmitz ML. Sci Rep. 2017 Jun 14;7(1):3547. doi: 10.1038/s41598-017-03708-7. 10.1038/s41598-017-03708-7 PubMed 28615693
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