pAT172_AAV_p3_PE7-N
(Plasmid
#251106)
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PurposeEncodes the N terminal of the PE7 prime editor in an AAV expression plasmid
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 251106 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepuc19
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Vector typeAAV, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePE7 C-terminal
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Alt nameprime editor 7
- Promoter p3
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer gcggcctctagatcagggta
- 3′ sequencing primer gcggCCATGGCACACAAAAA
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAT172_AAV_p3_PE7-N was a gift from Gerald Schwank (Addgene plasmid # 251106 ; http://n2t.net/addgene:251106 ; RRID:Addgene_251106) -
For your References section:
RNA-LNP-mediated in vivo prime editing corrects disease phenotypes in a mouse model of citrullinemia type I. Talas A, Ioannidi EI, Weber Y, Haenggi T, Kulcsar PI, Zurcher N, Faccin E, Moon WJ, Kissling L, Villiger EA, Matsushita M, Rothgangl T, Janjuha S, Lin PJC, Poms M, Muramatsu H, Vadovics M, Reichmuth A, Kopf M, Pardi N, Tam YK, Haberle J, Schwank G. Sci Transl Med. 2026 May 13;18(849):eaec7274. doi: 10.1126/scitranslmed.aec7274. Epub 2026 May 13. 10.1126/scitranslmed.aec7274 PubMed 42127219