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pMTLYN1C PtcdA::mNeonGreen
(Plasmid #251224)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251224 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pMTL-YN1C
  • Backbone size w/o insert (bp) 6064
  • Total vector size (bp) 7246
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PtcdA::mNeonGreen
  • Species
    C. difficile
  • Insert Size (bp)
    1182
  • Promoter tcdA

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ctgatgagtgtcctttatgtaagg
  • 3′ sequencing primer taacgccagggttttccc
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMTLYN1C PtcdA::mNeonGreen was a gift from Aimee Shen (Addgene plasmid # 251224 ; http://n2t.net/addgene:251224 ; RRID:Addgene_251224)
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