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DENV_3ED3_C4I
(Plasmid #251250)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251250 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET-15b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DENV_3ED3
  • Species
    Dengue virus serotype 3
  • Tags / Fusion Proteins
    • 6xHis, 11 aa linker, thrombin cleavage site (N terminal on backbone)
    • 4xIle with a GG linker (C terminal on insert)

Cloning Information

  • Cloning method Other

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DENV_3ED3_C4I was a gift from Yutaka Kuroda (Addgene plasmid # 251250 ; http://n2t.net/addgene:251250 ; RRID:Addgene_251250)

  • For your References section:

    Anti-Dengue ED3 Long-Term Immune Response With T-Cell Memory Generated Using Solubility Controlling Peptide Tags. Islam MM, Miura S, Hasan MN, Rahman N, Kuroda Y. Front Immunol. 2020 Mar 17;11:333. doi: 10.3389/fimmu.2020.00333. eCollection 2020. 10.3389/fimmu.2020.00333 PubMed 32256488
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