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DENV_3ED3_C5K
(Plasmid #251253)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251253 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET-15b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DENV_3ED3
  • Species
    Dengue virus serotype 3
  • Tags / Fusion Proteins
    • 6xHis, 11 aa linker, thrombin cleavage site (N terminal on backbone)
    • 5xLys with a GG linker (C terminal on insert)

Cloning Information

  • Cloning method Other

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DENV_3ED3_C5K was a gift from Yutaka Kuroda (Addgene plasmid # 251253 ; http://n2t.net/addgene:251253 ; RRID:Addgene_251253)

  • For your References section:

    Solubility Controlling Peptide Tags of Opposite Charges Generate a Bivalent Immune Response Against Dengue ED3 Serotypes 3 and 4. Rahman N, Miura S, Okawa M, Kibria MG, Islam MM, Kuroda Y. Front Immunol. 2021 Jun 11;12:671590. doi: 10.3389/fimmu.2021.671590. eCollection 2021. 10.3389/fimmu.2021.671590 PubMed 34177912
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