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JEV-ED3_C5I
(Plasmid #251260)

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This material is available to academics and nonprofits only. Currently unavailable outside the U.S.
Item Catalog # Description Quantity Price (USD)
Plasmid 251260 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET-15b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    JEV-ED3_C5I
  • Species
    Japanese encephalitis virus
  • Tags / Fusion Proteins
    • 6xHis, 11 aa linker, thrombin cleavage site (N terminal on backbone)
    • 5xIle with a GG linker (C terminal on insert)

Cloning Information

  • Cloning method Other

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    JEV-ED3_C5I was a gift from Yutaka Kuroda (Addgene plasmid # 251260 ; http://n2t.net/addgene:251260 ; RRID:Addgene_251260)

  • For your References section:

    Neutralizing antibodies against the Japanese encephalitis virus are produced by a 12 kDa E. coli- expressed envelope protein domain III (EDIII) tagged with a solubility-controlling peptide. Islam MD, Islam MM, Inoue A, Yesmin S, Brindha S, Yoshizue T, Tsurui H, Kurosu T, Kuroda Y. Vaccine. 2025 May 22;56:127143. doi: 10.1016/j.vaccine.2025.127143. Epub 2025 Apr 22. 10.1016/j.vaccine.2025.127143 PubMed 40267616
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