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H1N1-PR8_RBD
(Plasmid #251261)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251261 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET-15b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    H1N1-PR8_RBD
  • Species
    H1N1-PR8 influenza virus
  • Tag / Fusion Protein
    • 6xHis, 11 aa linker, thrombin cleavage site (N terminal on backbone)

Cloning Information

  • Cloning method Other

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    H1N1-PR8_RBD was a gift from Yutaka Kuroda (Addgene plasmid # 251261 ; http://n2t.net/addgene:251261 ; RRID:Addgene_251261)

  • For your References section:

    Design and Escherichia coli Expression of a Natively Folded Multi-Disulfide Bonded Influenza H1N1-PR8 Receptor-Binding Domain (RBD). Tu T, Rathnayaka T, Kato T, Mizutani K, Saotome T, Noguchi K, Kidokoro SI, Kuroda Y. Int J Mol Sci. 2024 Apr 1;25(7):3943. doi: 10.3390/ijms25073943. 10.3390/ijms25073943 PubMed 38612753
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