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pAAV-EF1α-GRAB_PGE2mut
(Plasmid #251356)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251356 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 5119
  • Total vector size (bp) 7029
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PGE2-insensitive sensor GRAB_PGE2mut (control sensor)
  • Alt name
    PGE2mut
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1917
  • Promoter EF1α

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.06.01.657218 for the bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-EF1α-GRAB_PGE2mut was a gift from Yulong Li (Addgene plasmid # 251356 ; http://n2t.net/addgene:251356 ; RRID:Addgene_251356)

  • For your References section:

    A genetically encoded fluorescent sensor for monitoring spatiotemporal prostaglandin E2 dynamics in vivo. Wang L, Yang Y, Deng F, Yan Y, Li Y. bioRxiv [Preprint]. 2025 Jun 3:2025.06.01.657218. doi: 10.1101/2025.06.01.657218. 10.1101/2025.06.01.657218 PubMed 40501671
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