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SFFV-Cas9-2A-mCherry
(Plasmid #251397)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251397 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    LentiV
  • Backbone size w/o insert (bp) 7455
  • Total vector size (bp) 11555
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cas9
  • Alt name
    S. pyogenes CRISPR-Cas9
  • Species
    Synthetic
  • Insert Size (bp)
    4100
  • Promoter SFFV
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • mCherry (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Christopher Vakoc (Addgene plasmid # 108100).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid was created by swapping the EFS promoter of the LentiV_Cas9_Puro vector (Addgene #108100) with the SFFV promoter and then replacing the Puro with mCherry using the In-Fusion cloning strategy (Takara).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SFFV-Cas9-2A-mCherry was a gift from Junwei Shi (Addgene plasmid # 251397 ; http://n2t.net/addgene:251397 ; RRID:Addgene_251397)

  • For your References section:

    CRISPR-based functional genomics for dissecting therapeutic dependency in primary acute myeloid leukemia samples. Cao Z, Yu S, Peng J, Barrett DR, Liu Y, Sussman JH, Chen C, Thadi A, Liu L, Alikarami F, Xu J, Carroll MP, Tan K, Bernt KM, Shi J. Mol Cell. 2026 Mar 5;86(5):968-985.e7. doi: 10.1016/j.molcel.2026.02.003. Epub 2026 Feb 26. 10.1016/j.molcel.2026.02.003 PubMed 41759529
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