pSC101-hfq2
(Plasmid
#251407)
-
PurposeExpresses WT hfq gene with an altered RBS sequence
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 251407 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepSC101
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Spectinomycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pSC101-hfq2 was a gift from Dokyun Na (Addgene plasmid # 251407 ; http://n2t.net/addgene:251407 ; RRID:Addgene_251407) -
For your References section:
Rational Design of High-Efficiency Synthetic Small Regulatory RNAs and Their Application in Robust Genetic Circuit Performance Through Tight Control of Leaky Gene Expression. Ren J, Nong NT, Lam Vo PN, Lee HM, Na D. ACS Synth Biol. 2024 Oct 18;13(10):3256-3267. doi: 10.1021/acssynbio.4c00323. Epub 2024 Sep 18. 10.1021/acssynbio.4c00323 PubMed 39294875