pET-28a(+)-N-T-M50
(Plasmid #251408)

• Purpose: Expresses human medin as a fusion protein in E. coli cytoplasm cleavable by TEV protease post purification.
• Depositing Lab: Michele Vendruscolo
• Publication: Roy Chowdhury et al Commun Chem. 2026 Apr 8;9(1):194. doi: 10.1038/s42004-026-01950-7.

Backbone

• Vector backbone: pET-28a(+)
• Backbone manufacturer: GenScript Biotech, United Kingdom
• Backbone size w/o insert (bp): 5224
• Total vector size (bp): 606
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: The growth strain is NEB® 5-alpha Competent E. coli which is a derivative of DH5alpha. It is T1 phage resistant and endA deficient for high-quality plasmid preparations.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Medin
• Species: H. sapiens (human)
• Insert Size (bp): 606
• Entrez Gene: MFGE8 (a.k.a. BA46, EDIL1, HMFG, HsT19888, MFG-E8, MFGM, OAcGD3S, SED1, SPAG10, hP47)
• Promoter: T7
• Tags / Fusion Proteins:
  • 6xHis (N terminal on insert)
  • NT*Flsp (N terminal on insert)
  • TEV protease recognition site (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The gene insert for the 6xHis-NT*Flsp-TEVp-Medin fusion protein was cloned into a pET-28a(+) plasmid backbone upon user request by and was purchased from GenScript Biotech, United Kingdom.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry