PurposeBacterial expression for structure determination; may not be full ORF
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25147||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7328
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Alt namePDB: 3I3T
Alt nameubiquitin specific peptidase 21
SpeciesH. sapiens (human)
Insert Size (bp)1065
Entrez GeneUSP21 (a.k.a. USP16, USP23)
/ Fusion Protein
- His (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
PDB: 3I3T. http://www.thesgc.org/structures/3I3T/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:USP21 (3I3T) was a gift from Cheryl Arrowsmith (Addgene plasmid # 25147 ; http://n2t.net/addgene:25147 ; RRID:Addgene_25147)