pSC06
(Plasmid #251508)

• Purpose: Payload integration, Bxb1 attP
• Depositing Lab: James Carothers
• Publication: Conjugation-based Serine Recombinase-Assisted Genome Engineering (cSAGE) Enables Bioproduction in Non-model Bacteria (unpublished)

Backbone

• Vector backbone: pR6K
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Pir1
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: BBa_J23119::mRFP

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Elmore, Joshua R., et al. "High-throughput genetic engineering of nonmodel and undomesticated bacteria via iterative site-specific genome integration." Science Advances 9.10 (2023): eade1285.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Alternative name: pMG065

Usage/Comments: This plasmid is a Bxb1 attP-containing payload integration vector expressing mRFP from a constitutive BBa_J23119 promoter with a Bujard RBS. It is designed for chromosomal integration via Bxb1 recombination into the corresponding attB site within a cSAGE landing pad strain. Upon conjugative co-delivery with the Bxb1 integrase plasmid (pSC02), this plasmid integrates in the Bxb1 attB site. It is useful for reporter benchmarking, promoter characterization, or serving as a template for constructing custom Bxb1-compatible payloads. Please visit https://doi.org/10.64898/2026.04.17.717921 for bioRxiv preprint.