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pSR77
(Plasmid #251524)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251524 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pRSFDuet™-1
  • Backbone manufacturer
    SnapGene
  • Backbone size w/o insert (bp) 3829
  • Total vector size (bp) 9293
  • Modifications to backbone
    MCS-1: Cas6, Cas8, Cas7, Cas5 MCS-2: Repeat-Spacer-Repeat, CasDinG
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Cas6
  • Alt name
    csf5
  • Species
    Pseudomonas oleovorans
  • Insert Size (bp)
    711
  • GenBank ID
  • Promoter T7

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Cas8
  • Alt name
    csf1
  • Species
    Pseudomonas oleovorans
  • Insert Size (bp)
    724
  • GenBank ID
  • Promoter T7

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    Cas7
  • Alt name
    csf2
  • Species
    Pseudomonas oleovorans
  • Insert Size (bp)
    1043
  • GenBank ID
  • Promoter T7

Cloning Information for Gene/Insert 3

Gene/Insert 4

  • Gene/Insert name
    Cas5
  • Alt name
    csf3
  • Insert Size (bp)
    668
  • GenBank ID
  • Promoter T7

Cloning Information for Gene/Insert 4

Gene/Insert 5

  • Gene/Insert name
    CRISPR-RNA
  • Alt name
    Repeat-Spacer-Repeat
  • Species
    Pseudomonas oleovorans
  • Insert Size (bp)
    90
  • GenBank ID
  • Promoter T7

Cloning Information for Gene/Insert 5

Gene/Insert 6

  • Gene/Insert name
    CasDinG
  • Alt name
    csf4
  • Species
    Pseudomonas oleovorans
  • Insert Size (bp)
    2157
  • GenBank ID
  • Promoter T7

Cloning Information for Gene/Insert 6

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

All-in-one plasmid expressing:
- Type IV-AI Cas5,6,7,8
- Repeat-Spacer-Repeat CRISPR-RNA (Filler spacer can be exchanged by BseRI golden gate cloning for desired target RNA)
- CasDinG

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSR77 was a gift from Lennart Randau (Addgene plasmid # 251524 ; http://n2t.net/addgene:251524 ; RRID:Addgene_251524)

  • For your References section:

    Visualization of Type IV-A1 CRISPR-mediated repression of gene expression and plasmid replication. Sanchez-Londono M, Rust S, Hernandez-Tamayo R, Gomes-Filho JV, Thanbichler M, Randau L. Nucleic Acids Res. 2024 Nov 11;52(20):12592-12603. doi: 10.1093/nar/gkae879. 10.1093/nar/gkae879 PubMed 39380487
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