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pET28a-Dob2
(Plasmid #251540)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251540 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5302
  • Total vector size (bp) 9023
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    We recommend transforming this plasmid in BAP1 cells.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Dob2
  • Species
    Nocardia ninae
  • Insert Size (bp)
    3780
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.05.24.655970 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-Dob2 was a gift from Emily Balskus (Addgene plasmid # 251540 ; http://n2t.net/addgene:251540 ; RRID:Addgene_251540)

  • For your References section:

    Chemical capture of diazo metabolites reveals biosynthetic hydrazone oxidation. Pfeifer K, Van Cura D, Wu KJY, Balskus EP. Nature. 2026 Feb 4. doi: 10.1038/s41586-025-10079-x. 10.1038/s41586-025-10079-x PubMed 41639443
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