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p3XFLAG-CMV7.1-hRpn2 (916-953)
(Plasmid #251557)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251557 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    p3xFLAG-CMV-7.1
  • Backbone size w/o insert (bp) 4696
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PSMD1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    114
  • Entrez Gene
    PSMD1 (a.k.a. P112, Rpn2, S1)
  • Promoter CMV forward
  • Tag / Fusion Protein
    • 3xFlag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer CMV forward
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3XFLAG-CMV7.1-hRpn2 (916-953) was a gift from Kylie Walters (Addgene plasmid # 251557 ; http://n2t.net/addgene:251557 ; RRID:Addgene_251557)

  • For your References section:

    A High Affinity hRpn2-Derived Peptide That Displaces Human Rpn13 from Proteasome in 293T Cells. Lu X, Liu F, Durham SE, Tarasov SG, Walters KJ. PLoS One. 2015 Oct 14;10(10):e0140518. doi: 10.1371/journal.pone.0140518. eCollection 2015. 10.1371/journal.pone.0140518 PubMed 26466095
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