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pDD0257 pCS2-sfCherry11Mx4-10aa-zH2B-SV40NLS-sfGFP-NPLnls
(Plasmid #251772)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251772 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCS2
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2B
  • Species
    D. rerio (zebrafish)
  • Entrez Gene
    hist2h2l (a.k.a. fj05g08, wu:fj05g08, zgc:73093)
  • Promoter CMV IE94
  • Tags / Fusion Proteins
    • 4x sfCherry3C small fragments, NLS (SV40) (N terminal on insert)
    • NLS (nucleoplasmin), sfGFP (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TGCAggatcccatcgattc
  • 3′ sequencing primer tacgactcactatagttctagaGGctcgag
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDD0257 pCS2-sfCherry11Mx4-10aa-zH2B-SV40NLS-sfGFP-NPLnls was a gift from Dave Dingal (Addgene plasmid # 251772 ; http://n2t.net/addgene:251772 ; RRID:Addgene_251772)

  • For your References section:

    Identification and engineering of highly functional potyviral proteases in cells using co-evolutionary models. Lim Suan MB, Ziegler C, Syed Z, Yedavalli AS, Nagineni J, Raposo R, Tunikipati A, Kaur J, Morcos F, Dingal DP. Nat Commun 17, 3257 (2026). 10.1038/s41467-026-69961-5
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