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pDM1514-Plin
(Plasmid #252025)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252025 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pDM1514
  • Vector type
    Amoebal Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    plin
  • Species
    Dictyostelium discoideum
  • Insert Size (bp)
    1139
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unspecified (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDM1514-Plin was a gift from Thierry Soldati (Addgene plasmid # 252025 ; http://n2t.net/addgene:252025 ; RRID:Addgene_252025)

  • For your References section:

    Membrane microdomains are crucial for Mycobacterium marinum EsxA-dependent membrane damage, escape to the cytosol, and infection. Perret A, Bosmani C, Leuba F, Eblighatian K, Gueho A, Raykov L, Hanna N, Soldati T. Sci Adv. 2026 Jan 2;12(1):eady0812. doi: 10.1126/sciadv.ady0812. Epub 2026 Jan 1. 10.1126/sciadv.ady0812 PubMed 41477822
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