pDM1513-D4H*
(Plasmid
#252026)
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PurposeSafe haven knock-in plasmid for the act5 locus, N-terminal GFP tagging of the gene Vps32
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252026 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepDM1513
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Vector typeAmoebal Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameD4H*
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SpeciesUnspecified
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Insert Size (bp)328
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unspecified (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDM1513-D4H* was a gift from Thierry Soldati (Addgene plasmid # 252026 ; http://n2t.net/addgene:252026 ; RRID:Addgene_252026) -
For your References section:
Membrane microdomains are crucial for Mycobacterium marinum EsxA-dependent membrane damage, escape to the cytosol, and infection. Perret A, Bosmani C, Leuba F, Eblighatian K, Gueho A, Raykov L, Hanna N, Soldati T. Sci Adv. 2026 Jan 2;12(1):eady0812. doi: 10.1126/sciadv.ady0812. Epub 2026 Jan 1. 10.1126/sciadv.ady0812 PubMed 41477822