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pcDNA3 LiON
(Plasmid #252093)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252093 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LiON (Labile iron and Oxygen Notifier)
  • Species
    Synthetic
  • Insert Size (bp)
    2679

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer CMV promoter
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is also associated with the following publication:

Maeda A, Moroishi T. (2026) Protocol to assess intracellular labile iron and oxygen at single-cell resolution using a genetically encoded ratiometric reporter. STAR Protocols, 2026; 7.
PMID: 42118663. DOI: https://doi.org/10.1016/j.xpro.2026.104561

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 LiON was a gift from Toshiro Moroishi (Addgene plasmid # 252093 ; http://n2t.net/addgene:252093 ; RRID:Addgene_252093)

  • For your References section:

    In vivo visualization of bioactive iron and oxygen using LiON, the labile iron and oxygen notifier. Maeda A, Nita A, Sashiyama S, Yoshitomo S, Jepkosgei KJ, Xu Y, Arima Y, Nakayama KI, Araki K, Moroishi T. Cell Rep Methods. 2026 May 8:101431. doi: 10.1016/j.crmeth.2026.101431. 10.1016/j.crmeth.2026.101431 PubMed 42105746
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