MSCVhygro-SNF2H(K211R)-3xT7
(Plasmid #252289)

• Purpose: Retroviral vector for stable expression of enzyme dead mutant of human SNF2H-3xT7 in mammalian cell lines
• Depositing Lab: Kai Ge
• Depositing Organization: National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK)
  Located in United States of America
• Publication: ISWI remodeler facilitates lineage-determining transcription factor-dependent BAF binding to chromatin during cell differentiation (unpublished)

Backbone

• Vector backbone: MSCVhygro
• Backbone size w/o insert (bp): 7000
• Total vector size (bp): 10452
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SNF2H
• Species: H. sapiens (human)
• Insert Size (bp): 3471
• Mutation: Changed Lysine 211 to Arginine
• GenBank ID: NM_003601.4
• Entrez Gene: SMARCA5 (a.k.a. ISWI, SNF2H, WCRF135, hISWI, hSNF2H)
• Promoter: GAG
• Tag / Fusion Protein:
  • 3xT7 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: HpaI (not destroyed)
• 5′ sequencing primer: pLXSN 5'
• 3′ sequencing primer: MSCV-Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry