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pCW57.1puro-3xT7-C/EBPa
(Plasmid #252291)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252291 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pCW57.1puro
  • Backbone size w/o insert (bp) 7800
  • Total vector size (bp) 8874
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cebpa
  • Species
    M. musculus (mouse)
  • GenBank ID
    NM_001287514
  • Entrez Gene
    Cebpa (a.k.a. C/ebpalpha, CBF-A, Cebp)
  • Promoter TRE
  • Tag / Fusion Protein
    • 3xT7 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HpaI (not destroyed)
  • 5′ sequencing primer LNCX
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCW57.1puro-3xT7-C/EBPa was a gift from Kai Ge (Addgene plasmid # 252291 ; http://n2t.net/addgene:252291 ; RRID:Addgene_252291)
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