pCW57.1puro-3xT7-d127PPARg
(Plasmid #252292)

• Purpose: Lentiviral vector for inducible expression of 3xT7-d127PPARg in mammalian cell lines
• Depositing Lab: Kai Ge
• Depositing Organization: National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK)
  Located in United States of America
• Publication: ISWI remodeler facilitates lineage-determining transcription factor-dependent BAF binding to chromatin during cell differentiation (unpublished)

Backbone

• Vector backbone: pCW57.1puro
• Backbone size w/o insert (bp): 7800
• Total vector size (bp): 9040
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Pparg
• Species: M. musculus (mouse)
• Insert Size (bp): 1300
• Mutation: Deleted the N- terminal 127 amino acids of PPARg
• GenBank ID: NM_001308352
• Entrez Gene: Pparg (a.k.a. Nr1c3, PPAR-gamma, PPAR-gamma2, PPARgamma, PPARgamma2)
• Promoter: TRE
• Tag / Fusion Protein:
  • 3xT7 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HpaI (not destroyed)
• 5′ sequencing primer: LNCX

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry