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pLV-EF1a-IRES-puro-SNF2H-dTAG-2XHA
(Plasmid #252293)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252293 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pLV-EF1a-IRES-puro
  • Backbone size w/o insert (bp) 7900
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SNF2H
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3471
  • GenBank ID
    NM_003601.4
  • Entrez Gene
    SMARCA5 (a.k.a. ISWI, SNF2H, WCRF135, hISWI, hSNF2H)
  • Promoter EF-1a
  • Tag / Fusion Protein
    • dTAG-2XHA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HpaI (not destroyed)
  • 5′ sequencing primer EF-1a-F
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-EF1a-IRES-puro-SNF2H-dTAG-2XHA was a gift from Kai Ge (Addgene plasmid # 252293 ; http://n2t.net/addgene:252293 ; RRID:Addgene_252293)
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