pENTR221-TurquoiseGL
(Plasmid
#252336)
-
PurposeGateway shuttle vector with mTurquoiseGL.
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252336 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepDONR221
-
Backbone manufacturerThermo Fisher (Invitrogen)
- Backbone size w/o insert (bp) 4761
-
Vector typeGateway shuttle vector
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namemTurquoiseGL
-
SpeciesSynthetic
-
Insert Size (bp)709
- Promoter None
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer m13_F
- 3′ sequencing primer m13_R
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cloned by a BP reaction after PCR amplification of mTurquoiseGL.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pENTR221-TurquoiseGL was a gift from James Conway (Addgene plasmid # 252336 ; http://n2t.net/addgene:252336 ; RRID:Addgene_252336) -
For your References section:
Red-to-Near-Infrared Fluorescence Resonance Energy Transfer Reporters for Dual Imaging Applications. Tran VT, Ek F, Yassine H, Behera R, Hytonen VP, Conway JRW. Small Methods. 2026 Jul 12:e70828. doi: 10.1002/smtd.70828. 10.1002/smtd.70828 PubMed 42438328