pEF.DEST51-Aquamarine
(Plasmid
#252342)
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PurposeTransient expression of Aquamarine.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252342 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepEF-DEST51
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Backbone manufacturerThermo Fisher (Invitrogen)
- Backbone size w/o insert (bp) 7464
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAquamarine
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SpeciesSynthetic
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Insert Size (bp)717
- Promoter EF1a
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Tag
/ Fusion Protein
- V5; 6xHis (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer EF1a_F
- 3′ sequencing primer BGH_R
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cloned by an LR reaction with pENTR221-Aquamarine and pEF-DEST51.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF.DEST51-Aquamarine was a gift from James Conway (Addgene plasmid # 252342 ; http://n2t.net/addgene:252342 ; RRID:Addgene_252342) -
For your References section:
Red-to-Near-Infrared Fluorescence Resonance Energy Transfer Reporters for Dual Imaging Applications. Tran VT, Ek F, Yassine H, Behera R, Hytonen VP, Conway JRW. Small Methods. 2026 Jul 12:e70828. doi: 10.1002/smtd.70828. 10.1002/smtd.70828 PubMed 42438328