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pENTR2b-Eevee-S6K
(Plasmid #252350)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252350 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pENTR2b
  • Backbone manufacturer
    Thermo Fisher (Invitrogen)
  • Backbone size w/o insert (bp) 3755
  • Vector type
    Gateway shuttle vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Eevee-S6K
  • Species
    Synthetic
  • Insert Size (bp)
    2310
  • Promoter None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer attL1_F
  • 3′ sequencing primer attL2_R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The Eevee-S6K FRET reporter was first described in Komatsu, N., et al. (2011). "Development of an optimized backbone of FRET biosensors for kinases and GTPases." Mol Biol Cell 22(23): 4647-4656.http://www.ncbi.nlm.nih.gov/pubmed/21976697

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Restriction cloned from 3568NES Eevee-S6K using SalI/EcoRI, then ligated into pENTR2b using compatible ends from XhoI/EcoRI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR2b-Eevee-S6K was a gift from James Conway (Addgene plasmid # 252350 ; http://n2t.net/addgene:252350 ; RRID:Addgene_252350)

  • For your References section:

    Red-to-Near-Infrared Fluorescence Resonance Energy Transfer Reporters for Dual Imaging Applications. Tran VT, Ek F, Yassine H, Behera R, Hytonen VP, Conway JRW. Small Methods. 2026 Jul 12:e70828. doi: 10.1002/smtd.70828. 10.1002/smtd.70828 PubMed 42438328
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