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pPB.DEST-Eevee-S6K
(Plasmid #252351)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252351 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pPB.DEST
  • Backbone size w/o insert (bp) 8986
  • Vector type
    Mammalian Expression ; Transposon-based stable expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Eevee-S6K
  • Species
    Synthetic
  • Insert Size (bp)
    2310
  • Promoter CAG

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The Eevee-S6K FRET reporter was first described in Komatsu, N., et al. (2011). "Development of an optimized backbone of FRET biosensors for kinases and GTPases." Mol Biol Cell 22(23): 4647-4656.http://www.ncbi.nlm.nih.gov/pubmed/21976697

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloned by an LR reaction with pENTR2b-Eevee-S6K and pPB.DEST.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPB.DEST-Eevee-S6K was a gift from James Conway (Addgene plasmid # 252351 ; http://n2t.net/addgene:252351 ; RRID:Addgene_252351)

  • For your References section:

    Red-to-Near-Infrared Fluorescence Resonance Energy Transfer Reporters for Dual Imaging Applications. Tran VT, Ek F, Yassine H, Behera R, Hytonen VP, Conway JRW. Small Methods. 2026 Jul 12:e70828. doi: 10.1002/smtd.70828. 10.1002/smtd.70828 PubMed 42438328
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