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pMMLV-RT-PS
(Plasmid #252404)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252404 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pQE-81L
  • Backbone manufacturer
    Qiagen
  • Backbone size w/o insert (bp) 4723
  • Total vector size (bp) 6670
  • Modifications to backbone
    insert is located between Sph I and Hind III sites
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MMLV-RT
  • Alt name
    gag-pol
  • Alt name
    Pr180
  • Alt name
    reverse transcriptase
  • Species
    Moloney murine leukemia virus
  • Insert Size (bp)
    1947
  • Mutation
    ∆1-23, Q84A, H204R, V223H, T306K, F309N, D524N
  • GenBank ID
    NC_001501.1 (357..5573)
  • Promoter T5
  • Tag / Fusion Protein
    • 6xHis-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SphI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer TTGTGAGCGGATAACAA
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Peter Stoilov

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMMLV-RT-PS was a gift from Melissa Jurica (Addgene plasmid # 252404 ; http://n2t.net/addgene:252404 ; RRID:Addgene_252404)
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