pcDNA3_SaCas9-NNG
(Plasmid
#252406)
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PurposeExpression of SaCas9-NNG in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252406 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepcDNA3.1
- Total vector size (bp) 8743
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSaCas9-NNG
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SpeciesSynthetic
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Insert Size (bp)3156
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MutationN985A/R991A/E782K/L800R/T927K/K929N/N968R/N986S/I1017F/A1021S
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Tag
/ Fusion Protein
- HA-SV40 NLS
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.21203/rs.3.rs-4619764/v1 for preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3_SaCas9-NNG was a gift from Osamu Nureki (Addgene plasmid # 252406)