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pAL0004
(Plasmid #252719)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252719 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    LT3GEPIR
  • Backbone size w/o insert (bp) 10147
  • Total vector size (bp) 10272
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    shOTUD5-2
  • gRNA/shRNA sequence
    TAGACAGTATGAAGAAAAACAA
  • Species
    H. sapiens (human)
  • Entrez Gene
    OTUD5 (a.k.a. DUBA, MCAND)
  • Promoter TRE3G

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer catggtcctgctggagttcgtg
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAL0004 was a gift from James Brenton & Carlos Caldas (Addgene plasmid # 252719 ; http://n2t.net/addgene:252719 ; RRID:Addgene_252719)
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