pVK-U
(Plasmid
#252744)
-
Purpose(Empty Backbone) Geminivirus derived-CRISPR/Cas9 plasmid cloning of up to four gRNAs. Encodes GFP and kanamycin resistance.
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252744 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepGEF-U
- Backbone size (bp) 17180
-
Modifications to backboneAddition of a kanamycin resistance gene fused to GFP
-
Vector typePlant Expression, CRISPR
-
Selectable markersKanamycin
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)EPI300
-
Copy numberHigh Copy
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pVK-U was a gift from Humberto Prieto (Addgene plasmid # 252744 ; http://n2t.net/addgene:252744 ; RRID:Addgene_252744) -
For your References section:
Transgene-Free Editing of PPO2 in Elite Potato Cultivar YAGANA for Reduced Postharvest Browning. Grbich, M.; Muñoz, M.; Zúñiga, G.E.; Valdovinos, G.; Acha, G.; Vergara, R.; Mora, R.; Olivares, F.; Olmedo, B.; Prieto, H.. Agronomy 2026, 16, 216. https://doi.org/10.3390/agronomy16020216 10.3390/agronomy16020216
