Skip to main content
Addgene
Open Menu

pET28-SpBglA-2
(Plasmid #252910)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252910 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pET28
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    6-phospho-beta-glucosidase
  • Species
    Klebsiella pneumoniae subsp. pneumoniae
  • Insert Size (bp)
    1398
  • GenBank ID
    AE005672.3
  • Promoter T7
  • Tag / Fusion Protein
    • His-tagged (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7 promoter
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.06.15.659766 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-SpBglA-2 was a gift from David Kwan (Addgene plasmid # 252910 ; http://n2t.net/addgene:252910 ; RRID:Addgene_252910)

  • For your References section:

    A high sensitivity strategy to screen NAD(P)H-dependent oxidoreductase activity by coupled enzyme cascade. Ghosh T, Sicheri J, Kwan DH. bioRxiv 2025.06.15.659766 10.1101/2025.06.15.659766
Related items: