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pSL137
(Plasmid #252925)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252925 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    ColE1 ori_ampR_AMβ1 ori_ermR (modified from pECGMC3)
  • Vector type
    Bacterial Expression
  • Selectable markers
    Erythromycin resistance (10 ug/mL) in Lactococcus lactis

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    menD
  • Alt name
    PnisA_RBSLl4_menD
  • Species
    Synthetic
  • Promoter PnisA

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.09.24.678173 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSL137 was a gift from Caroline Ajo-Franklin (Addgene plasmid # 252925 ; http://n2t.net/addgene:252925 ; RRID:Addgene_252925)

  • For your References section:

    Synthetic microbial co-cultures for modular bioelectronic sensing in diverse environments. Li S, Zhu D, Saha K, Kundu BB, Sonkusale S, Britton RA, Ajo-Franklin CM. Nat Biotechnol (2026). 10.1038/s41587-026-03075-7
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