pSL180
(Plasmid
#252939)
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PurposeArsenite sensing in the sender Escherichia coli "Marionette" strain with sRBSarsR3 for use in e-COSENS
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252939 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonep15A ori_cmR
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namearsR, menD
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Alt namePJ109_sRBSarsR3_arsR_ParsOC2_SarJ_RBSEc4_menD
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SpeciesSynthetic
- Promoter PJ109, ParsOC2
Cloning Information
- Cloning method Golden Gate
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.09.24.678173 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSL180 was a gift from Caroline Ajo-Franklin (Addgene plasmid # 252939 ; http://n2t.net/addgene:252939 ; RRID:Addgene_252939) -
For your References section:
Synthetic microbial co-cultures for modular bioelectronic sensing in diverse environments. Li S, Zhu D, Saha K, Kundu BB, Sonkusale S, Britton RA, Ajo-Franklin CM. Nat Biotechnol (2026). 10.1038/s41587-026-03075-7