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pET28-tpiA
(Plasmid #252950)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252950 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET28
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    triosephosphate isomerase
  • Species
    E. coli
  • GenBank ID
    X00617.1
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • His-tagged (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.06.15.659766 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-tpiA was a gift from David Kwan (Addgene plasmid # 252950 ; http://n2t.net/addgene:252950 ; RRID:Addgene_252950)

  • For your References section:

    A high sensitivity strategy to screen NAD(P)H-dependent oxidoreductase activity by coupled enzyme cascade. Ghosh T, Sicheri J, Kwan DH. bioRxiv 2025.06.15.659766 10.1101/2025.06.15.659766
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