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3x-FLAG-MGMT
(Plasmid #253098)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253098 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pDEST-302
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MGMT
  • Species
    H. sapiens (human)
  • Entrez Gene
    MGMT
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3x-FLAG-MGMT was a gift from Harish Vasudevan (Addgene plasmid # 253098 ; http://n2t.net/addgene:253098 ; RRID:Addgene_253098)

  • For your References section:

    Multiplexed epigenetic memory editing using CRISPRoff sensitizes glioblastoma to chemotherapy. Lin K, Zou C, Hubbard A, Sengelmann S, Goudy L, Wang IC, Sharma R, Pak J, Foster K, Ozawa T, de Groot JF, Phillips J, Vasudevan HN, Raleigh DR, Marson A, Murthy N, Gilbert LA, Berger MS, Liu SJ. Neuro Oncol. 2025 Jul 30;27(6):1443-1457. doi: 10.1093/neuonc/noaf055. 10.1093/neuonc/noaf055 PubMed 39998382
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