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pUC19_CoV-2_frD_ΔE/GFP Δ68
(Plasmid #253121)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253121 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fragment D2 SARS-CoV-2 ΔE-GFPΔ6Δ8
  • Species
    SARS-CoV-2

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Detailed explanation of CLEVER reverse genetics methodology in https://doi.org/10.7554/eLife.89035

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19_CoV-2_frD_ΔE/GFP Δ68 was a gift from Thomas Klimkait (Addgene plasmid # 253121 ; http://n2t.net/addgene:253121 ; RRID:Addgene_253121)

  • For your References section:

    Rapid cloning-free mutagenesis of new SARS-CoV-2 variants using a novel reverse genetics platform. Kipfer ET, Hauser D, Lett MJ, Otte F, Urda L, Zhang Y, Lang CMR, Chami M, Mittelholzer C, Klimkait T. Elife. 2023 Nov 21;12:RP89035. doi: 10.7554/eLife.89035. 10.7554/eLife.89035 PubMed 37988285
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