pcDNA3.1 CMV-F DS-Cav1 ΔCT
(Plasmid
#253156)
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PurposeRespiratory syncytial virus (RSV) F glycoprotein, stabilized in pre-fusion conFormation, DS-Cav1 deltaCT; CMV-driven expression plasmid
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Depositing Lab
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Depositing OrganizationUniversity of Basel
Located in Switzerland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 253156 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRSV preF
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SpeciesRespiratory syncytial virus (RSV)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A Y146S mutation was found in the insert, this does not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1 CMV-F DS-Cav1 ΔCT was a gift from Thomas Klimkait (Addgene plasmid # 253156 ; http://n2t.net/addgene:253156 ; RRID:Addgene_253156) -
For your References section:
Chimeric SARS-CoV-2 vaccine vectors stably incorporating the functional glycoprotein of VSV. Kipfer ET, Schon J, Chami M, Finke S, Beer M, Hoffmann D, Otte F, Hauser D. iScience. 2026 Sep 16;29(10):117521. doi: 10.1016/j.isci.2026.117521. eCollection 2026 Oct 16. 10.1016/j.isci.2026.117521 PubMed 42781491