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pUC19_CoV-2_ frD2_ΔE67 3* F-P2A-N DS Cav1 ΔCT
(Plasmid #253167)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253167 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CoV-2 frD2 with RSV preF
  • Species
    SARS-CoV-2, Respiratory syncytial virus (RSV)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

detailed explanation of CLEVER reverse genetics methodology in DOI: 10.7554/eLife.89035

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19_CoV-2_ frD2_ΔE67 3* F-P2A-N DS Cav1 ΔCT was a gift from Thomas Klimkait (Addgene plasmid # 253167 ; http://n2t.net/addgene:253167 ; RRID:Addgene_253167)

  • For your References section:

    Rapid cloning-free mutagenesis of new SARS-CoV-2 variants using a novel reverse genetics platform. Kipfer ET, Hauser D, Lett MJ, Otte F, Urda L, Zhang Y, Lang CMR, Chami M, Mittelholzer C, Klimkait T. Elife. 2023 Nov 21;12:RP89035. doi: 10.7554/eLife.89035. 10.7554/eLife.89035 PubMed 37988285
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