pBAD-SepA
(Plasmid
#253228)
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PurposeExpresses SepA from Shigella flexneri - wild type AA sequence
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 253228 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepBAD
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Backbone manufacturerThermo Fisher
- Backbone size w/o insert (bp) 4157
- Total vector size (bp) 8249
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSepA secreted autotransporter protease
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SpeciesShigella flexneri
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Insert Size (bp)4092
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Entrez GenesepA (a.k.a. CP0070)
- Promoter pBAD
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Tag
/ Fusion Protein
- None
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pBAD_F
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBAD-SepA was a gift from James Fleckenstein (Addgene plasmid # 253228 ; http://n2t.net/addgene:253228 ; RRID:Addgene_253228)