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pBAD-SepA
(Plasmid #253228)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253228 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pBAD
  • Backbone manufacturer
    Thermo Fisher
  • Backbone size w/o insert (bp) 4157
  • Total vector size (bp) 8249
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SepA secreted autotransporter protease
  • Species
    Shigella flexneri
  • Insert Size (bp)
    4092
  • Entrez Gene
    sepA (a.k.a. CP0070)
  • Promoter pBAD
  • Tag / Fusion Protein
    • None

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD-SepA was a gift from James Fleckenstein (Addgene plasmid # 253228 ; http://n2t.net/addgene:253228 ; RRID:Addgene_253228)