pmScarlet-i-N1-hDNM2DN
(Plasmid
#253468)
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Purposemammalian expression of DNM2DN-Sca
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Depositing Lab
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Depositing OrganizationRIKEN Center for Biosystems Dynamics Research, Kobe
Located in Japan -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 253468 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepEGFP-N1 (GenBank: U55762.1)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman dynamin2(K44A) fused with mScarlet-i
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SpeciesH. sapiens (human)
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Mutationdynamin2: K44A
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that the depositor's annotated reference file has minor differences compared to the Addgene verified sequence. Please refer to the the Addgene sequence for the most accurate result.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmScarlet-i-N1-hDNM2DN was a gift from Yasushi Okada (Addgene plasmid # 253468 ; http://n2t.net/addgene:253468 ; RRID:Addgene_253468)