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LuxCDE/pRSETb
(Plasmid #253526)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253526 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pRSETb
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LuxCDE
  • Species
    Synthetic
  • Promoter T7

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer atgactaaaaaaatttcattcattattaacggccag
  • 3′ sequencing primer tcaactatcaaacgcttcggttaagct
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LuxCDE/pRSETb was a gift from Takeharu Nagai (Addgene plasmid # 253526 ; http://n2t.net/addgene:253526 ; RRID:Addgene_253526)

  • For your References section:

    Autonomous multicolor bioluminescence imaging in bacteria, mammalian, and plant hosts. Kusuma SH, Kakizuka T, Hattori M, Nagai T. Proc Natl Acad Sci U S A. 2024 Oct 8;121(41):e2406358121. doi: 10.1073/pnas.2406358121. Epub 2024 Oct 2. 10.1073/pnas.2406358121 PubMed 39356665
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